Research

We are a multi-disciplinary research group focused on developing quantitative biomarkers for non-invasive, real-time assessments of tissue structure and function to diagnose disease or trauma and guide therapies.

Label-free multiphoton microscopy can provide a variety of non-invasive quantitative biomarkers of cell metabolism, collagen fiber organization, and tissue composition. By quantifying the naturally present fluorescence of NADH and FAD without our cells, we can non-invasively measure dynamic changes in the metabolism of live cells, tissues, and organisms. Second harmonic generation (SHG) imaging can also provide a non-destructive measure of 3D collagen organization within tissues. While acquiring two-photon excited fluorescence (TPEF) and SHG signals, we can also measure coherent anti-Stokes Raman scattering (CARS), which provides images of a tissue’s protein and lipid composition. We combine these imaging methods with advanced image analysis approaches to quantify the orientation, morphology, and fractal organization of different cell and tissue features.

The following selected press releases provide an overview of our techniques and how we are using them to solve various biomedical research and health-related problems:

Feb 2019: NIH Funds Interdisciplinary Team Pursuing Better Mitochondrial Disease Diagnosis
Feb 2019: Academic Minute: Chronic Skin Wounds
Nov 2018: Researchers Develop New Method to Diagnose, Monitor Chronic Wounds
Nov 2018: NIBIB-funded researchers use non-invasive imaging technique to diagnose, monitor chronic wounds
Aug 2018: Collaborative Research Earns Recognition for Graduate Students Studying Mitochondrial Disease
Jun 2018: Biomedical Engineering Professor Receives Department of Defense Funding for Wound-Healing Research
Apr 2018: Imaging Method Evaluates Cell Functional Changes and Wound Healing
Sep 2017: Biomedical Engineering Professor Receives $1.7 Million NIH Grant to Continue Wound Healing Research
Feb 2017: New Study Shows Tissue Healing Response Following a Heart Attack
Oct 2015: NIH Grant Will Advance Study of Chronic Wound Biomarkers

Our research primarily focuses on skin wound healing and aging applications in which we can integrate label-free multiphoton microscopy with advanced image analysis and mechanical testing. However, we are always interested in collaborating with researchers in other areas where non-invasive multiphoton microscopy or advanced image analysis of tissue microstructure can aid in scientific discovery and advancements in medicine. Below are a list of our active research collaborations that have produced publications or secured significant funding:


For more information, please contact Dr. Kyle Quinn (kpquinn AT uark.edu).